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Sample Analysis

Imanis Life Sciences specializes in virological and reporter gene assays for the detection of live virus, gene transfer, gene expression, and more. We offer several standard sample analysis services:

Click below to learn more about each of our sample analysis services or to request a quote. Contact us at support@imanislife.com for more information.

  • RCV testing

    Replication-competent virus (RCV) testing


    Lentiviral vector-transduced cells should be used at Biosafety level 2. However, several institutes allow in vivo experiments to be downgraded to Biosafety level 1 if the cell have been tested and found negative for replication-competent virus (RCV). Imanis offers RCV testing for lentivirus using an enzyme-linked immunosorbent assay (ELISA) directed against the lentiviral p24 capsid protein. This rapid and highly-sensitive assay is the most widely-accepted test for detecting RCV. The limit of detection for our assay is 12.5 pg/mL of p24.

    Pricing


    The cost for RCV testing is $425 per sample with bulk discounts available for order of 5 or more samples. The negative control is included in the price and is not counted as an additional sample.

    Sample submission


    Samples should be submitted as culture supernatants from cells grown for at least two weeks after lentiviral vector transduction. Culture supernatants collected earlier may yield false positive results. A negative (media only) control should also be submitted for each sample being tested.

    For more information about sample submission please download our RCV sample submission form or contact us at support@imanislife.com.

  • Lentivirus titration

    Lentivirus titration by qPCR


    Having an accurate titer for lentiviral vector stocks is important for optimizing transductions. Imanis can titer your lentiviral vectors using the same precise quantitative PCR (qPCR)-based method used for our extensive catalog of lentiviral vectors. Unlike traditional p24 ELISAs that fail to distinguish between functional and defective lentiviral particles, qPCR of integrated lentiviral genome copies exclusively measures the number of functional transducing units (TU/mL), giving you a more accurate titer to begin your studies.

    Our standard qPCR titration targets the WPRE, a post-transcriptional regulatory sequence commonly included in lentiviral vectors. For titration of lentiviral vectors that do not contain a WPRE, please refer to our contract service page.

    Pricing


    The cost for lentiviral vector titering is $425 per lentiviral vector stock. For more information or to submit samples for titration, please contact us at support@imanislife.com.

  • NIS reporter assays

    Iodine uptake assay to test sodium iodide symporter (NIS) function


    Imanis provides in vitro testing to confirm NIS expression prior to in vivo delivery of viral vectors or cell lines. Samples are tested using our standard iodine (125I) uptake assay.

    Pricing


    Pricing varies depending on the type of samples being assayed. For more information, please contact us at support@imanislife.com

  • Request a Quote

What people say about Imanis

Our group has, and continues to, use NIS as a noninvasive reporter for cell transplantation studies in mice and in pigs. Imanis has provided expert technical and analytical support for this research, and has allowed us to publish our research in high impact journals, including Science Translational Medicine..

– Dr. Raymond Hickey, Mayo Clinic

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