Call Now

Anti-rat NIS antibody (affinity purified)

Description: Polyclonal antibody to rat NIS 

Antigen: Synthetic peptide corresponding to residues 603-618 of the rat sodium iodide symporter

Detects both the native and denatured forms of rNIS. This antibody recognizes an intracellular C-terminal epitope that is conserved between rat and mouse NIS

Host Species: Rabbit

Isotype: Not applicable. Polyclonal affinity purified antibody.

Concentration: 1 mg/mL

Species reactivity: Rat, mouse 


Tested Applications:

  • Flow cytometry (1:500 dilution)
  • Immunofluorescence (1:500 dilution) 
  • Immunohistochemistry (1:500 dilution)
  • Western Blotting (1:500 to 1:1,000 dilution) 

Selected Product References:

1. Levy et al. Proc. Natl. Acad. Sci. USA. 1997. 94:5568-5573
2. De la Vieja et al. J. Cell Sci. 2004. 117:677-687
3. Nicola et al. Am. J. Physiol. Cell Physiol. 2009. 296:C654-C662
4. Tazebay et al. Nat. Med. 2000. 6:871-878
5. Paroder-Belenitsky et al. Proc. Natl. Acad. Sci. USA. 2011. 108:17933-38.

 

Immunofluorescence staining for rat NIS expression:

HelaH1 cells stably-expressing (left) rat NIS or (right) human NIS were permeabilized and stained with anti-rat NIS antibody (1:500 dilution) followed by an Alexa Fluor 594-conjugated anti-rabbit secondary antibody and Hoechst 33342 to stain nuclei. Cell photos were taken at 200X magnification.

Anti-Rat NIS antibody staining IF IHC Western

Immunoblotting for rat NIS protein:

Total protein (10 mg) from HeLaH1 cells stably-expressing rat (lane 1), dog (lane 2), pig (lane 3), or rhesus (lane 4) NIS were subjected to SDS-PAGE and western blot analysis using anti-rat NIS antibody (1:3,000 dilution) and HRP-conjugated anti-rabbit secondary antibody. The top band (~75-90 kDa) represents the hyperglycosylated form of rNIS, while the bottom band (~60 kDa) represents the hypoglycosylated form of rNIS. Data from Dr. Nancy Carrasco's (Yale University) laboratory.

western blot rat NIS protein antibody carrasco Imanis

 

Why Choose Imanis?

With quality reagents, services, and support, we have you covered at all stages of your study. Our expert scientists can help you select the best reagents for your needs and offer tips to optimize your experiments and achieve superior results. Our wide-range of reporter gene and oncolytic virus reagents undergo rigorous quality control testing so you can be confident in the quality of the products you receive. And with our competitive prices, you can complete your study for less.

  • “Our group has, and continues to, use NIS as a noninvasive reporter for cell transplantation studies in mice and in pigs. Imanis has provided expert technical and analytical support for this research, and has allowed us to publish our research in high impact journals, including Science Translational Medicine.” – Dr. Raymond Hickey, Mayo Clinic

Use Fewer Animals

Our Reduction Campaign

At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...

Use Fewer Animals

Our Reduction Campaign

At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...