Call Now

Vaccinia vTF7-3

  • 0.25 mL / Standard (REA006) $ 450

Ready to use vaccinia TF7-3 virus

  • Description: Replication competent vaccinia virus encoding the bacteriophage T7 RNA polymerase as a transgene. 
  • Strain: Vaccinia (Western Reserve WR strain)
  • Transgene: Bacteriophage T7 RNA polymerase
  • Biosafety Level: 2
  • Reference: Fuerst et al., PNAS. 1986, 83(21): 8122-8126.
  • Titer: Standard (clarified supernatant, 3e5 to 1e6 TCID50/ml).
  • Propagation methodInoculate producer cell (e.g. Vero or BHK-21) at MOI of 0.02 for 2-3 hours. Harvest supernatant when 80-90% of cell monolayer is showing cytopathic effects (CPE).
  • Proposed Use: Ready to use viral stock for rescue of recombinant viruses from plasmid DNA encoding viral genes driven by a T7 RNA polymerase promoter.
license from USDA is required to use this virus. Click here to apply for the license.

      Use Fewer Animals

      Our Reduction Campaign

      At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...

      Use Fewer Animals

      Our Reduction Campaign

      At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...