Call Now

Anti-human NIS antibody SJ1

Description: Rabbit polyclonal antibody to human NIS (hNIS)

Antigen: MBP/C-terminal portion of the human NIS (amino acid 468-643) fusion protein (MBP-hNIS).

Reference: Jhiang SM. Endocrinology. 1998; 139(10):4416-9. PMID:9751526

Species: Rabbit

Reactivity: Human, Rhesus macaque

Isotype: Not applicable

Tested Applications:

  • Western blot (1:3000 to 1:5000)
  • Immunofluorescence (1:500 to 1:3000)
  • Immunohistochemistry (1:2000 to 1:5000)
  • Flow cytometry analysis (1:2000 to 1:3000)
NoteUsers are strongly advised to determine the optimal dilution of antibody to use for their specific applications.
Western Blot: 
Lane 1: Total membrane protein from Mel624-hNIS-Neo cells (20 ug). Lane 2: Total membrane protein from control murine CT26.WT-mNIS cells (20 ug). Anti-hNIS Ab was used at 1:2000 dilution. The expected size of hyperglycosylated hNIS is 75-90 kDa (top band) and hypoglycosylated hNIS is 60-65 kDa (bottom band).

 

 

 
Immunofluorescence staining:
Intact or permeabilized Mel624-hNIS-Neo cells were stained with anti hNIS SJ1 antibody (1:500 dilution), followed by incubation of Alexa 594 conjugated anti-rabbit antibody.

 

Immunohistochemical staining:

 

NIS expression in (A) human thyroid or (B) VSV-mIFNβ-NIS virus treated mouse tumor xenograft. Antigen retrieval was performed on paraffin embedded sections prior to incubation with anti hNIS SJ1 antibody (1:2000). Counterstain is hematoxylin.

 

Flow cytometry:

Flow cytometry of human thyroid cell line SW579 (high sodium iodide symporter expression) stained with anti-human NIS antibody SJ1

Human thyroid cell, SW579 were fixed and stained with anti hNIS SJ1 (1:2000), 1h on ice. Cells were then stained with Alexa 594 conjugated anti-rabbit antibody for 30 min on ice.
 

 

 

Why Choose Imanis?

With quality reagents, services, and support, we have you covered at all stages of your study. Our expert scientists can help you select the best reagents for your needs and offer tips to optimize your experiments and achieve superior results. Our wide-range of reporter gene and oncolytic virus reagents undergo rigorous quality control testing so you can be confident in the quality of the products you receive. And with our competitive prices, you can complete your study for less.

  • “Our group has, and continues to, use NIS as a noninvasive reporter for cell transplantation studies in mice and in pigs. Imanis has provided expert technical and analytical support for this research, and has allowed us to publish our research in high impact journals, including Science Translational Medicine.” – Dr. Raymond Hickey, Mayo Clinic

Use Fewer Animals

Our Reduction Campaign

At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...

Use Fewer Animals

Our Reduction Campaign

At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...