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Anti-human NIS antibody clone VJ1

Description: Monoclonal antibody to human NIS clone VJ1

Antigen: Human sodium iodide symporter.

Recognizes the extracellular domains of hNIS (ref. 2). Epitope recognition by the anti-hNIS antibody clone is located between amino acid 272 and 515 located within the last three extracellular loops (shaded area).

Extracellular domain of sodium iodide symporter (NIS) recognized by anti-NIS antibody VJ1

Species: Mouse

Isotype: IgG1

Species reactivity: Human

Cross reactivity: Sheep (ref. 1). This antibody does not cross react with rat or mouse NIS

Tested Applications:

  • Flow cytometry. Use at 1:50 dilution
  • Immunofluorescence. Use at 1:10 to 1:50 dilution. 
  • This antibody VJ1 detects the native form of NIS and does not recognize the denatured form.

Selected Product References:

1. Hombach-Klonisch S. Mol Cell Endocrinol. 2013; 10;367(1-2):98-108. PMID: 23291342
2. Pohlenz J. et al. J Clin Endocrinol Metab. 2000; 85 (7):2366-9. PMID: 10902780
3. Marsee DK. et al. Cancer Gene Therapy. 2004; 11, 121–127. PMID: 14730332
4. Hou P. et. al. PLoS One. 2009; 10;4(7):e6200. PMID: 19593429
5. Higuchi T. et. al. J Nucl Med. 2009; 50(7):1088-94. PMID: 19525455
6. Hou P. J Clin Endocrinol Metab. 2010;95(2):820-8. PMID: 20008023

Flow cytometry to detect human NIS expression on surface of lentiviral transduced human cell lines stably expressing human NIS

Tissue: Lentiviral transduced 293 cells expressing NIS.  Type: Fresh cells Stain: Secondary antibody Alexa 555.   Dilution: 1:50.

Flow cytometry of sodium iodide symporter (NIS) expressing HEK293 and Mel624 cell lines with anti-NIS antibody VJ1

Immunofluorescence staining for human NIS expression:

Tissue: Lentiviral transduced Mel624-hNIS-Neo cells stably expressing NIS, 200x
Type: Cytospin of cells on glass slide.  Stain: Secondary antibody Alexa 555 with Hoechst 33342.  Dilution: 1:50

Immunofluorescent staining of sodium iodide symporter (NIS) expressing Mel624 cells stained with anti-NIS Vj1 antibody using Alexa 555 secondary

Tissue: Cryosection of tumor infected with an oncolytic virus expressing NIS, showing areas of NIS expressing areas, 200x.  Type: OCT cryosection .  Stain: Secondary antibody Alexa 555 with Hoechst 33342.  Dilution: 1:50Immunofluorescent staining of sodium iodide symporter (NIS) expressing oncolytic virus infection stained with anti-NIS VJ1 antibody using Alexa 555 secondary

 

Why Choose Imanis?

With quality reagents, services, and support, we have you covered at all stages of your study. Our expert scientists can help you select the best reagents for your needs and offer tips to optimize your experiments and achieve superior results. Our wide-range of reporter gene and oncolytic virus reagents undergo rigorous quality control testing so you can be confident in the quality of the products you receive. And with our competitive prices, you can complete your study for less.

  • “Our group has, and continues to, use NIS as a noninvasive reporter for cell transplantation studies in mice and in pigs. Imanis has provided expert technical and analytical support for this research, and has allowed us to publish our research in high impact journals, including Science Translational Medicine.” – Dr. Raymond Hickey, Mayo Clinic

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Use Fewer Animals

Our Reduction Campaign

At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...