pLV-Ub-mouse NIS-P2A-Neo
Amount | 20 ug |
Full Name | pHR'-SIN-Ub-mNIS-P2A-Neo |
Promoter | Ubiquitin (Ub) promoter |
Transgene | Mouse sodium iodide symporter (mNIS) Neomycin resistance (Neo) |
Linker | Self-cleaving peptide P2A |
-
Plasmid Map
-
Validation
NIS Expression
HeLaH1 cells were transduced with LV-Ub-mNIS-Neo and selected with G418. NIS mediated I-125 uptake was assayed in the presence or absence of KClO4, a competitive inhibitor of iodine uptake.
-
Proposed Use
For generation of lentiviral vector stocks encoding mouse sodium iodide symporter (mNIS) under G418 selection in a triple transfection protocol.