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Imanis Life Sciences



Amount20 ug
Full NamepHR'-SIN-Ub-Luc2-P2A-Puro
PromoterUbiquitin (Ub) promoter
TransgeneFirefly luciferase (Luc2) and puromycin resistance (Puro)
LinkerSelf-cleaving peptide P2A
  • Plasmid Map

  • Validation

    Luciferase assay

    HeLaH1 cells were transduced with LV-Ub-Luc2-Puro and selected with puromycin. A luciferase assay was performed and imaged with a Xenogen IVIS Spectrum.

  • Proposed Use

    For generation of lentiviral vector stocks encoding firefly luciferase (Luc2) under puromycin selection in a triple transfection protocol.

What people say about Imanis

Our group has, and continues to, use NIS as a noninvasive reporter for cell transplantation studies in mice and in pigs. Imanis has provided expert technical and analytical support for this research, and has allowed us to publish our research in high impact journals, including Science Translational Medicine..

– Dr. Raymond Hickey, Mayo Clinic

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