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pLV-Ub-Luc2-P2A-Puro

  • 20 ug (DNA1071) $ 660
  • Name of plasmid: pLV-Ub-Luc2-P2A-Puro
  • DescriptionpHR'-SIN-Ub-Luc2-P2A-Puro plasmid DNA
  • Amount of DNA: 20 ug
  • Promoter: Ubiquitin (Ub) promoter for constitutive gene expression in numerous cell types.
  • Transgenes: Firefly luciferase (Luc2) and puromycin resistance (Puro)
  • Linker: Self-cleaving peptide P2A
  • Proposed Use: For generation of lentiviral vector stocks encoding dual transgenes firefly luciferase and puromycin resistance in a triple transfection protocol.

Plasmid Map:

 

Transgene Validation: HeLaH1 cells were transduced with LV-Ub-Luc2-Puro and selected with puromycin. A luciferase assay was performed and imaged with a Xenogen IVIS Spectrum.

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At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...

Use Fewer Animals

Our Reduction Campaign

At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...