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pLV-Ub-human NIS-P2A-Puro

Amount20 ug
Full NamepHR'-SIN-Ub-hNIS-P2A-Puro
PromoterUbiquitin (Ub) promoter
TransgeneHuman sodium iodide symporter (hNIS) and puromycin resistance (Puro)
LinkerSelf-cleaving peptide P2A
  • Plasmid Map

  • Validation

    Iodine Uptake Assay (NIS Function)


    HelaH1 cells were transduced with LV-Ub-hNIS-P2A-Puro  and selected with puromycin. NIS mediated 125I uptake was assayed in the presence or absence of KClO4, a competitive inhibitor of iodine uptake.

  • Proposed Use

    For generation of lentiviral vector stocks encoding human sodium iodide symporter (hNIS) under puromycin selection in a triple transfection protocol.

What people say about Imanis

Our group has, and continues to, use NIS as a noninvasive reporter for cell transplantation studies in mice and in pigs. Imanis has provided expert technical and analytical support for this research, and has allowed us to publish our research in high impact journals, including Science Translational Medicine..

– Dr. Raymond Hickey, Mayo Clinic

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