|Promoter||Ubiquitin (Ub) promoter|
|Transgene||Human sodium iodide symporter (hNIS) and puromycin resistance (Puro)|
|Linker||Self-cleaving peptide P2A|
Iodine Uptake Assay (NIS Function)
HelaH1 cells were transduced with LV-Ub-hNIS-P2A-Puro and selected with puromycin. NIS mediated 125I uptake was assayed in the presence or absence of KClO4, a competitive inhibitor of iodine uptake.
For generation of lentiviral vector stocks encoding human sodium iodide symporter (hNIS) under puromycin selection in a triple transfection protocol.