pLV-Ub-human NIS-P2A-Puro
| Amount | 20 ug |
| Full Name | pHR'-SIN-Ub-hNIS-P2A-Puro |
| Promoter | Ubiquitin (Ub) promoter |
| Transgene | Human sodium iodide symporter (hNIS) and puromycin resistance (Puro) |
| Linker | Self-cleaving peptide P2A |
-
Plasmid Map
-
Validation
NIS Expression
HelaH1 cells were transduced with LV-Ub-hNIS-P2A-Puro and selected with puromycin. NIS mediated I-125 uptake was assayed in the presence or absence of KClO4, a competitive inhibitor of iodine uptake. -
Proposed Use
For generation of lentiviral vector stocks encoding human sodium iodide symporter (hNIS) under puromycin selection in a triple transfection protocol.