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pLV-SFFV-Rhesus NIS

  • 20 ug (DNA1037) $ 1,150
  • Name of plasmid: pLV-SFFV-RhNIS
  • DescriptionpHR'-SIN-SFFV-Rhesus NIS plasmid DNA
  • Amount of DNA: 20 ug
  • Promoter: SFFV spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in rodent and other animal cells.
  • Transgene: Rhesus sodium iodide symporter (RhNIS)
  • Proposed Use: For generation of lentiviral vector stocks encoding Rhesus NIS in a triple transfection protocol.

Plasmid Map:

plasmid map, RhNIS

 

Transgene Validation: Uptake assay of LV-RhNIS transduced cells in the presence or absence of KClO4, an inhibitor of NIS-mediated 125I  uptake.

uptake, RhNIS

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At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...

Use Fewer Animals

Our Reduction Campaign

At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...