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pLV-SFFV-Luc2-P2A-Neo

Amount20 ug
Full NamepHR'-SIN-SFFV-Luc2-P2A-Neo
PromoterSpleen focus-forming virus (SFFV) promoter
TransgeneFirefly luciferase (Luc2) and neomycin resistance (Neo)
LinkerSelf-cleavage peptide P2A
  • Plasmid Map

    plasmid map, Luc2 neomycin

  • Validation

    Luciferase assay


    luciferase assay, IVISHeLaH1 cells were transduced with LV-Luc2-Neo and selected with G418. A luciferase assay was performed and imaged with a Xenogen IVIS Spectrum.

  • Proposed Use

    For generation of lentiviral vector stocks encoding firefly luciferase (Luc2) under G418 selection in a triple transfection protocol.

What people say about Imanis

Our group has, and continues to, use NIS as a noninvasive reporter for cell transplantation studies in mice and in pigs. Imanis has provided expert technical and analytical support for this research, and has allowed us to publish our research in high impact journals, including Science Translational Medicine..

– Dr. Raymond Hickey, Mayo Clinic

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