|Promoter||Spleen focus-forming virus (SFFV) promoter|
|Transgene||Firefly luciferase (Luc2) and neomycin resistance (Neo)|
|Linker||Self-cleavage peptide P2A|
HeLaH1 cells were transduced with LV-Luc2-Neo and selected with G418. A luciferase assay was performed and imaged with a Xenogen IVIS Spectrum.
For generation of lentiviral vector stocks encoding firefly luciferase (Luc2) under G418 selection in a triple transfection protocol.