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pLV-SFFV-Luc2-P2A-Neo

  • 20 ug (DNA1033) $ 660
  • Name of plasmid: pLV-SFFV-Luc2-P2A-Neo
  • DescriptionpHR'-SIN-SFFV-Luc2-P2A-Neo plasmid DNA
  • Amount of DNA: 20 ug
  • Promoter: SFFV spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in rodent and other animal cells.
  • Transgenes: Firefly luciferase (Luc2) and neomycin resistance (Neo)
  • Linker: Self-cleaving peptide P2A
  • Proposed Use: For generation of lentiviral vector stocks encoding dual transgenes firefly luciferase and neomycin resistance in a triple transfection protocol.

Plasmid Map:

plasmid map, Luc2 neomycin

 

Transgene Validation: HeLaH1 cells were transduced with LV-Luc2-Neo and selected with G418. A luciferase assay was performed and imaged with a Xenogen IVIS Spectrum.

luciferase assay, IVIS

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Our Reduction Campaign

At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...

Use Fewer Animals

Our Reduction Campaign

At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...