|Promoter||Spleen focus-forming virus (SFFV) promoter|
|Transgene||Near-infrared fluorescent protein (iRFP; excitation: 690nm, emission: 713nm) and puromycin resistance (Puro)|
|Linker||Self-cleavage peptide P2A|
Flow cytometry for iRFP
LV-iRFP-Puro transduced (red) or control (grey) cells were fixed with paraformaldehyde and analyzed by flow cytometry.
For generation of lentiviral vector stocks encoding near-infrared fluorescent protein (iRFP) under puromycin selection in a triple transfection protocol.