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Imanis Life Sciences



Amount20 ug
Full NamepHR'-SIN-SFFV-iRFP-P2A-Puro
PromoterSpleen focus-forming virus (SFFV) promoter
TransgeneNear-infrared fluorescent protein (iRFP; excitation: 690nm, emission: 713nm) and puromycin resistance (Puro)
LinkerSelf-cleavage peptide P2A
  • Plasmid Map

    plasmid map iRFP puro

  • Validation

    Flow cytometry for iRFP

    flow cytometry iRFPLV-iRFP-Puro transduced (red) or control (grey) cells were fixed with paraformaldehyde and analyzed by flow cytometry.

  • Proposed Use

    For generation of lentiviral vector stocks encoding near-infrared fluorescent protein (iRFP) under puromycin selection in a triple transfection protocol.

What people say about Imanis

Our group has, and continues to, use NIS as a noninvasive reporter for cell transplantation studies in mice and in pigs. Imanis has provided expert technical and analytical support for this research, and has allowed us to publish our research in high impact journals, including Science Translational Medicine..

– Dr. Raymond Hickey, Mayo Clinic

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