pLV-SFFV-iRFP-P2A-Puro
| Amount | 20 ug |
| Full Name | pHR'-SIN-SFFV-iRFP-P2A-Puro |
| Promoter | Spleen focus-forming virus (SFFV) promoter |
| Transgene | Near-infrared fluorescent protein (iRFP; excitation: 690nm, emission: 713nm) Puromycin resistance (Puro) |
| Linker | Self-cleavage peptide P2A |
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Plasmid Map
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Validation
iRFP Expression
LV-iRFP-Puro transduced (red) or control (grey) cells were fixed with paraformaldehyde and analyzed by flow cytometry. -
Proposed Use
For generation of lentiviral vector stocks encoding near-infrared fluorescent protein (iRFP) under puromycin selection in a triple transfection protocol.