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pLV-SFFV-human NIS-Neo

  • 20 ug (DNA1006) $ 1,150
  • Name of plasmid: pLV-SFFV-human NIS-Neo
  • Description: pHR' SIN-SFFV-hNIS-IRES-Neo plasmid DNA
  • Amount of DNA: 20 ug
  • Promoter: SFFV spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in human, rodent, and other animal cells.
  • Transgenes: Human sodium iodide symporter (hNIS) and neomycin resistance (Neo)
  • Linker: IRES
  • Proposed Use: For generation of lentiviral vector stocks encoding human NIS and neomycin resistance in a triple transfection protocol.

Plasmid Map:

Transgene Validation: HeLaH1 cells were trasduced with LV-hNIS-Neo and selected with G418. NIS mediated 125I uptake was assayed in the presence or absence of KClO4, a competitive inhibitor of iodine uptake.

Uptake, hNIS, NIS

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Use Fewer Animals

Our Reduction Campaign

At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...