pLV-SFFV-hTyrosinase-PGK-Puro
| Amount | 20 ug |
| Full Name | pHR'-SIN-SFFV-hTYR-PGK-Puro |
| Promoter | Spleen focus-forming virus (SFFV) promoter Mouse phosphoglycerate kinase promoter (PGK) |
| Transgene | Human tyrosinase (hTYR) Puromycin resistance (Puro) |
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Plasmid Map
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Validation
Tyrosinase activity assay
Cells were transduced with LV-hTYR-PGK-Puro, selected with puromycin, and lysed. The lysates were incubated with L-DOPA, which in the presence of tyrosinase is converted to a colored compound with absorbance at 475 nm. B16F10 cells (endogenously express tyrosinase) and parental cells were used as controls. -
Proposed Use
For generation of lentiviral vector stocks encoding human tyrosinase (hTYR) under puromycin selection in a triple transfection protocol.