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pLV-SFFV-hTYR-PGK-Puro

  • 20 ug (DNA1057) $ 1,150
  • Name of plasmid: pLV-SFFV-hTYR-PGK-Puro
  • Description: pHR' SIN-SFFV-hTYR-PGK-Puro plasmid DNA
  • Amount of DNA: 20 ug
  • Promoter: SFFV: spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in human, rodent, and other animal cells. PGK: mouse phosphoglycerate kinase I promoter drives puromycin resistance for selection of transduced cells.
  • Transgenes: Human tyrosinase (hTYR) and puromycin resistance (Puro)
  • Proposed Use: For generation of lentiviral vector stocks encoding human tyrosinase and puromycin resistance in a triple transfection protocol.

Plasmid Map:

 plasmid map hTYR puro

 

Transgene Validation: Cells were transduced with LV-hTYR-PGK-Puro, selected with puromycin, and lysed. The lysates were incubated with L-DOPA, which in the presence of tyrosinase is converted to a colored compound with absorbance at 475 nm. B16F10 cells (endogenously express tyrosinase) and parental cells were used as controls. 

absorbance L-Dopa hTYR

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Use Fewer Animals

Our Reduction Campaign

At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...