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pLV-SFFV-HA-human NIS-PGK-Puro

  • 20 ug (DNA1019) $ 1,150
  • Name of plasmid: pLV-SFFV-HA-human NIS-PGK-Puro
  • Description: pHR' SIN-SFFV-HA-hNIS-PGK-Puro plasmid DNA
  • Amount of DNA: 20 ug
  • Promoter: SFFV: spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in human, rodent, and other animal cells. PGK: mouse phosphoglycerate kinase I promoter drives puromycin resistance for selection of transduced cells.
  • Transgenes: HA-tagged human sodium iodide symporter (HA-hNIS) and puromycin resistance (Puro)
  • Epitope tag: Human influenza hemagglutinin tag (HA)
  • Proposed Use: For generation of lentiviral vector stocks encoding human sodium iodide symporter and puromycin resistance in a triple transfection protocol.

Plasmid Map:

Transgene Validation: HeLaH1 cells were transduced with LV-HA-hNIS-PGK-Puro and selected with puromycin. NIS mediated 125I uptake was assayed in the presence or absence of KClO4, a competitive inhibitor of iodine uptake.

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At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...

Use Fewer Animals

Our Reduction Campaign

At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...