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pLV-PGK-mouse NIS-P2A-Neo

  • 20 ug (DNA1059) $ 1,150
  • Name of plasmid: pLV-PGK-mouse NIS-P2A-Neo
  • DescriptionpHR'-SIN-PGK-mNIS-P2A-Neo plasmid DNA
  • Amount of DNA: 20 ug
  • Promoter:  Phosphoglycerate kinase I (PGK) promoter for constitutive gene expression in numerous cell types. 
  • Transgenes: Mouse sodium iodide symporter (mNIS) and neomycin resistance (Neo)
  • Linker: Self-cleaving peptide P2A
  • Proposed Use: For generation of lentiviral vector stocks encoding mouse NIS and neomycin resistance in a triple transfection protocol.

Plasmid Map:

plasmid map pgk mnis puro

 

Transgene Validation: HeLaH1 cells were transduced with LV-PGK-mNIS-Neo and selected with G418. NIS mediated 125I uptake was assayed in the presence or absence of KClO4, a competitive inhibitor of iodine uptake.

uptake pgk mnis puro

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Use Fewer Animals

Our Reduction Campaign

At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...