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pLV-PGK-human NIS-P2A-Puro

  • 20 ug (DNA1058) $ 1,150
  • Name of plasmid: pLV-PGK-human NIS-P2A-Puro
  • Description: pHR' SIN-PGK-hNIS-P2A-Puro plasmid DNA
  • Amount of DNA: 20 ug
  • Promoter: Phosphoglycerate kinase I promoter (PGK) for constitutive gene expression in numerous cell types. 
  • Transgenes: Human sodium iodide symporter (hNIS) and puromycin resistance (Puro)
  • Linker: Self-cleavage peptide P2A
  • Proposed Use: For generation of lentiviral vector stocks encoding human NIS and puromycin resistance in a triple transfection protocol.

Plasmid Map:

plasmid map PGK hNIS puro

 

Transgene Validation: HelaH1 cells were transduced with LV-PGK-hNIS-P2A-Puro  and selected with puromycin. NIS mediated 125I uptake was assayed in the presence or absence of KClO4, a competitive inhibitor of iodine uptake.

uptake pgk hnis puro

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Use Fewer Animals

Our Reduction Campaign

At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...