pLV-PGK-humanNIS-P2A-Puro
Amount | 20 ug |
Full Name | pHR'-SIN-PGK-hNIS-P2A-Puro |
Promoter | Phosphoglycerate kinase promoter (PGK) |
Transgene | Human sodium iodide symporter (hNIS) Puromycin resistance (Puro) |
Linker | Self-cleavage peptide P2A |
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Plasmid Map
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Validation
NIS Expression
HelaH1 cells were transduced with LV-PGK-hNIS-P2A-Puro and selected with puromycin. NIS mediated I-125 uptake was assayed in the presence or absence of KClO4, a competitive inhibitor of iodine uptake.
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Proposed Use
For generation of lentiviral vector stocks encoding human NIS under puromycin selection in a triple transfection protocol.