|Promoter||Synthetic hybrid CAG promoter (CMV enhancer, chicken beta-Actin promoter and rabbit beta-Globin splice acceptor site)|
|Transgene||Firefly luciferase (Luc2) and puromycin resistance (Puro)|
|Linker||Self-cleaving peptide P2A|
HeLaH1 cells were transduced with LV-CAG-Luc2-Puro and selected with puromycin. A luciferase assay was performed and imaged with a Xenogen IVIS Spectrum.
For generation of lentiviral vector stocks encoding firefly luciferase (Luc2) under puromycin selection in a triple transfection protocol.