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HSV-GFP

  • 0.25 mL / Standard (OV3001) $ 550

Ready to use oncolytic HSV-GFP

  • Titer: Standard (1e7 TCID50/ml) 
  • Propagation method: Inoculate producer cells (e.g. Vero) at an MOI of 0.01 for 2-3 hours. Harvest culture supernatant and cells when 80-90% of the cell monolayer is showing cytopathic effects (CPE). Freeze-thaw 3 cycles and clarify the supernatant by low-speed centrifugation. Centrifuge the supernatant at 14,000 xg for 1h at 4°C. Resuspend the viral pellet in DPBS.
  • Proposed Use: Ready to use for generation of new viral stocks and in cell killing assays.
  • USDA permit required for shipping? No.

      Use Fewer Animals

      Our Reduction Campaign

      At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...

      Use Fewer Animals

      Our Reduction Campaign

      At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...