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VSV-hIFNβ-NIS

  • 0.25 mL / Standard (OV2003) $ 550
  • 1.0 mL / High (OV2016) $ 2,250

Ready to use oncolytic VSV-hIFNβ-NIS

  • Description: Live replication competent Indiana strain of Vesicular Stomatitis Virus (VSV) encoding human interferon beta (hIFNβ) inserted as an additional transcription unit upstream of the G glycoprotein and human sodium iodide symporter (NIS) upstream of L polymerase.
  • Strain: Lab adapted Indiana strain VSV
  • Transgene: hIFNβ upstream of G and NIS upstream of L
  • Biosafety Level: 2
  • Reference: Naik et al. Leukemia. 2012, 26(8): 1870-8. 
  • Titer: Standard (clarified supernatant, 3e5 to 1e6 TCID50/ml) or high titer purified supernatant (>1e9 TCID50/ml).
  • Propagation method:Inoculate producer cell (e.g. Vero or BHK) at MOI of 0.02 for 2-3 hours. Harvest supernatant when 80-90% of cell monolayer is showing cytopathic effects (CPE).
  • Proposed Use: Ready to use for generation of new viral stocks and in cell killing assays.
  • Recommended reagent: Anti-VSV antibody (REA005)
  • USDA permit required for shipping? Yes. Click here to fill out Application Form VS 16-3 to obtain a USDA APHIS VS 16-6 or 16-6A permit.

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      At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...

      Use Fewer Animals

      Our Reduction Campaign

      At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...