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Measles-GFP-NIS

  • 0.25 mL / Standard (OV1009) $ 550

Ready to use oncolytic MV-GFP-NIS 

  • Description: Live attenuated Edmonston strain of measles virus encoding GFP inserted as an additional transcription unit upstream of the nucleocapsid (N) gene and NIS downstream of the hemagglutinin (H) gene 
  • Strain: Edmonston vaccine lineage
  • Transgenes: Human NIS and GFP 
  • Biosafety Level: 1
  • Reference: Li et al. Cancer Gene Therapy (2010),17(8):550-558.
  • Titer: Standard (clarified supernatant, 3e5 to 1e6 TCID50/ml)
  • Propagation method: Inoculate producer cell (e.g. Vero) at MOI of 0.02 for 2-3 hours. Harvest cells when 80-90% of cell monolayer has formed syncytia. Freeze/thaw 2 cycles and freeze aliquots.
  • Proposed Use: Ready to use for generation of new viral stocks and in cell killing assays
  • USDA permit required for shipping? No.

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      At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...

      Use Fewer Animals

      Our Reduction Campaign

      At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...