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LV-eGFP-P2A-human NIS

LV-eGFP-P2A-hNIS purified high titer lentivirus stock

  • Description: LV-SFFV-eGFP-P2A-hNIS. 
  • This is a ready-to-use lentivirus preparation. The virus encodes the enhanced green fluorescent protein (eGFP) cDNA under control of the spleen focus-forming virus (SFFV) promoter linked to the human sodium iodide symporter (hNIS) cDNA via a P2A cleavage peptide (see below). The lentiviral vectors are self-inactivating (SIN) vectors in which the viral enhancer and promoter have been deleted. Transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization by replication competent viruses and enables regulated expression of the genes from the internal promoters without cis-acting effects of the LTR (Miyoshi et al. J Virol. 1998. 72:8150-8157).

  • Titer: 1e8 TU/ml in DMEM
  • Size: 0.25 mL or 1.0 mL
  • Promoter: SFFV spleen focus forming virus for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in human and rodent cells.
  • Transgene: Enhanced GFP linked to human NIS by a P2A sequence.

Transgene Validation

Fluorescence Expression: HeLaH1 cells were transduced with LV-eGFP-P2A-hNIS at the indicated MOIs. After 6 days fluorescence expression of the transduced (green) and untransducted parental HeLaH1 cells (grey) was analyzed by flow cytometry.

 

NIS Gene Expression: HeLaH1 cells were transduced with LV-eGFP-P2A-hNIS at the indicated MOIs. After 1 week 125I uptake by the cells was measured in the presence or absence of KClO4, an inhibitor of NIS-mediated 125I uptake.

Why Choose Imanis?

With quality reagents, services, and support, we have you covered at all stages of your study. Our expert scientists can help you select the best reagents for your needs and offer tips to optimize your experiments and achieve superior results. Our wide-range of reporter gene and oncolytic virus reagents undergo rigorous quality control testing so you can be confident in the quality of the products you receive. And with our competitive prices, you can complete your study for less.

  • “Our group has, and continues to, use NIS as a noninvasive reporter for cell transplantation studies in mice and in pigs. Imanis has provided expert technical and analytical support for this research, and has allowed us to publish our research in high impact journals, including Science Translational Medicine.” – Dr. Raymond Hickey, Mayo Clinic

Use Fewer Animals

Our Reduction Campaign

At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...

Use Fewer Animals

Our Reduction Campaign

At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...