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pLV-SFFV-SSTR2-PGK-Puro

  • 20 ug (DNA1055) $ 1,250
  • Name of plasmid: pLV-SFFV-SSTR2-PGK-Puro
  • Description: pHR' SIN-SFFV-SSTR2-PGK-Puro plasmid DNA
  • Amount of DNA: 20 ug
  • Promoter: SFFV: spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in human, rodent, and other animal cells. PGK: mouse phosphoglycerate kinase I promoter drives puromycin resistance for selection of transduced cells.
  • Transgenes: Human somatostatin receptor 2 (SSTR2) and puromycin resistance (Puro)
  • Proposed Use: For generation of lentiviral vector stocks encoding human somatostatin receptor 2 and puromycin resistance in a triple transfection protocol.

Plasmid Map:

plasmid map sstr2 puro

 

Transgene Validation: HeLaH1 cells were transduced with LV-SSTR2-PGK-Puro, selected with puromycin, and assayed for uptake of 111In-octreotide as a measure of SSTR2 activity. Parental HeLaH1 cells were used as a negative control.

uptake SSTR2 puro

 

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At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...

Use Fewer Animals

Our Reduction Campaign

At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...