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pLV-SFFV-HSVTK-PGK-Puro

  • 20 ug (DNA1053) $ 1,250
  • Name of plasmid: pLV-SFFV-HSVTK-PGK-Puro
  • Description: pHR' SIN-SFFV-HSVTK-PGK-Puro plasmid DNA
  • Amount of DNA: 20 ug
  • Promoter: SFFV spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in human, rodent, and other animal cells.
  • Transgenes: Herpes simplex virus thymidine kinase (HSVTK) and puromycin resistance
  • Proposed Use: For generation of lentiviral vector stocks encoding herpes simplex virus thymidine kinase and puromycin in a triple transfection protocol.

Plasmid Map: 

plasmid map hsvtk puro

 

Transgene Validation: HeLaH1 cells were transduced with LV-HSVTK-PGK-Puro, selected with puromycin, and assayed for uptake of 3H-penciclovir as a measure of HSVTK activity. Parental HeLaH1 cells were used as a negative control.

hsvtk puro uptake

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    Use Fewer Animals

    Our Reduction Campaign

    At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...