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pLV-SFFV-Luc2-P2A-human NIS

  • 20 ug (DNA1043) $ 1,150
  • Name of plasmid: pLV-SFFV-Luc2-P2A-human NIS
  • Description: pHR' SIN-SFFV-Luc2-P2A-hNIS plasmid DNA
  • Amount of DNA: 20 ug
  • Promoter: SFFV spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in human, rodent, and other animal cells.
  • Transgenes: Human sodium iodide symporter (hNIS) and firefly luciferase (Luc2)
  • Linker: Self-cleaving peptide P2A
  • Proposed Use: For generation of lentiviral vector stocks encoding firefly luciferase and human NIS in a triple transfection protocol.

Plasmid Map:

plasmid map luc2 hNIS

 

Transgene Validation:

Luciferase Assay: 104, 105, or 106 LV-Luc2-hNIS transduced cells were placed in wells of a 96-well plate and 0.3 mg of d-luciferin was added to the indicated wells. The plate was immediately imaged using a Xenogen IVIS Spectrum.

luciferase IVIS

Uptake Assay: NIS mediated 125I uptake of LV-Luc2-hNIS transduced cells was assayed in the presence or absence of KClO4, a competitive inhibitor of iodine uptake.

Uptake, hNIS, NIS

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Use Fewer Animals

Our Reduction Campaign

At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...