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pLV-SFFV-eGFP-P2A-human NIS

  • 20 ug (DNA1040) $ 1,150
  • Name of plasmid: pLV-SFFV-eGFP-P2A-human NIS
  • Description: pHR' SIN-SFFV-eGFP-P2A-hNIS plasmid DNA
  • Amount of DNA: 20 ug
  • Promoter: SFFV spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in human, rodent, and other animal cells,
  • Transgenes: Enhanced green fluorescent protein (eGFP) and human sodium iodide symporter (hNIS)
  • Linker: Self-cleavage peptide P2A
  • Proposed Use: For generation of lentiviral vector stocks encoding enhanced GFP and human NIS in a triple transfection protocol.

Plasmid Map:

plasmid map, eGFP hNIS

Transgene Validation:

Flow cytometry: LV-eGFP-hNIS transduced (green) or control (grey) cells were fixed with paraformaldehyde and analyzed by flow cytometry.

flow cytometry eGFP

Uptake Assay: NIS mediated 125I uptake of LV-eGFP-hNIS transduced cells was assayed in the presence or absence of KClO4, a competitive inhibitor of iodine uptake.

uptake

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At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...

Use Fewer Animals

Our Reduction Campaign

At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...