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pLV-SFFV-eGFP-PGK-Puro

  • 20 ug (DNA1022) $ 660
  • Name of plasmid: pLV-SFFV-eGFP-PGK-Puro
  • Description: pHR' SIN-SFFV-eGFP-PGK-Puro plasmid DNA
  • Amount of DNA: 20 ug
  • Promoter: SFFV: spleen focus forming virus promoter for high constitutive gene expression in numerous cell types. Unlike the CMV promoter, SFFV works very well in human, rodent, and other animal cells. PGK: mouse phosphoglycerate kinase I promoter drives puromycin resistance for selection of transduced cells.
  • Transgenes: Enhanced green fluorescent protein (eGFP) and puromycin (Puro)
  • Proposed Use: For generation of lentiviral vector stocks encoding enhanced GFP and puromycin in a triple transfection protocol.

Plasmid Map:

plasmid map, lentivector, eGFP

 

Transgene Validation: LV-eGFP-PGK-Puro transduced (green) or control (grey) cells were fixed with paraformaldehyde and analyzed by flow cytometry.

eGFP flow cytometry

Use Fewer Animals

Our Reduction Campaign

At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...

Use Fewer Animals

Our Reduction Campaign

At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...