- Frozen (CL018) $ 1,650
Cell type: Epithelial embryonic kidney
Transgenes: Human sodium iodide symporter (mNIS) and enhanced green fluorescent protein (eGFP)
Media: DMEM, 10% FBS, 1% Pen/Strep
Description: HEK293-hNIS-GFP is a polyclonal population of the human embryonic kidney HEK293 cell line transduced with a lentiviral vector LV-hNIS-IRES-eGFP encoding the human sodium iodide symporter (hNIS) cDNA under the spleen focus-forming virus (SFFV) promoter linked to the emerald green fluorescent protein (GFP) gene via an IRES (new Imanis item catalog #LV020, LV-eGFP-P2A-hNIS).
The lentiviral vector used is a self-inactivating (SIN) vector in which the viral enhancer and promoter has been deleted. Transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization by replication competent viruses and enables regulated expression of the genes from the internal promoters without cis-acting effects of the LTR (Miyoshi et al., J Virol. 1998).
Mycoplasma Testing: This cell line has been tested for mycoplasma contamination and is certified mycoplasma free.
In vitro: This high NIS expressing cell line can be used as a positive control for I-125 uptake assays to validate NIS expression in your lentiviral transduced cells.
GFP Fluorescence: Cell morphology and flow cytometry to confirm high GFP expression in cells.
NIS Function Assay (Iodine Uptake): Cells were incubated with 125I for 1h in the presence or absence of KClO4, an inhibitor of iodine uptake. Radioiodine concentrated within the cells was measured with a gamma counter.