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HEK293-hNIS-eGFP

Species: Human

Cell type: Epithelial embryonic kidney

Transgenes: Human sodium iodide symporter (mNIS) and enhanced green fluorescent protein (eGFP)

Media: DMEM, 10% FBS, 1% Pen/Strep

Description: HEK293-hNIS-GFP is a polyclonal population of the human embryonic kidney HEK293 cell line transduced with a lentiviral vector LV-hNIS-IRES-eGFP encoding the human sodium iodide symporter (hNIS) cDNA under the spleen focus-forming virus (SFFV) promoter linked to the emerald green fluorescent protein (GFP) gene via an IRES (new Imanis item catalog #LV020, LV-eGFP-P2A-hNIS).

The lentiviral vector used is a self-inactivating (SIN) vector in which the viral enhancer and promoter has been deleted. Transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization by replication competent viruses and enables regulated expression of the genes from the internal promoters without cis-acting effects of the LTR (Miyoshi et al., J Virol. 1998).

Mycoplasma Testing: This cell line has been tested for mycoplasma contamination and is certified mycoplasma free.

Recommended uses:

In vitro: This high NIS expressing cell line can be used as a positive control for I-125 uptake assays to validate NIS expression in your lentiviral transduced cells.

GFP Fluorescence: Cell morphology and flow cytometry to confirm high GFP expression in cells.

NIS Function Assay (Iodine Uptake): Cells were incubated with 125I for 1h in the presence or absence of KClO4, an inhibitor of iodine uptake. Radioiodine concentrated within the cells was measured with a gamma counter.

 

Why Choose Imanis?

With quality reagents, services, and support, we have you covered at all stages of your study. Our expert scientists can help you select the best reagents for your needs and offer tips to optimize your experiments and achieve superior results. Our wide-range of reporter gene and oncolytic virus reagents undergo rigorous quality control testing so you can be confident in the quality of the products you receive. And with our competitive prices, you can complete your study for less.

  • “Our group has, and continues to, use NIS as a noninvasive reporter for cell transplantation studies in mice and in pigs. Imanis has provided expert technical and analytical support for this research, and has allowed us to publish our research in high impact journals, including Science Translational Medicine.” – Dr. Raymond Hickey, Mayo Clinic

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Use Fewer Animals

Our Reduction Campaign

At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...