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Measles Virus

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Measles Virus representative image
Measles Virus representative image

Description

Type: (-) ssRNA enveloped paramyxovirus

Strain: Edmonston vaccine lineage (live attenuated)

Tropism: The Measles virus hemagglutinin (H) protein mediates binding to the following cellular receptors
  • CD46 receptor (most human tissue types)
  • SLAM receptor (immune-cells)
  • Nectin-4 receptor (throat epithelium)

  • Gene Map: (Arrows indicate transgene insertion sites)

     

    Proposed Uses

    Standard titer stocks are ready to use in cell killing assays or for generation of new viral stocks. High-titer purified stocks are ready for immediate use in in vivo studies.

    Protocol Tips

    Propagation: Inoculate producer cells (e.g. Vero cells) at an MOI of 0.02 for 2-3 hours. Collect the cells when 80-90% of the cell monolayer has formed syncytia. Lyse the cells by 2 freeze/thaw cycles in liquid nitrogen. Remove cell debris by centrifugation. Freeze and store aliquots at or below -70oC.

    Viral Titration: Titer virus by TCID50/mL endpoint dilution assay on Vero cells. Score CPE positive cells based on the presence of syncytia 4-5 days after inoculation.  

    Regulatory Information

    Biosafety Level: 1/2 (Check details on specific product pages)

    US shipping license required? No.

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    Use Fewer Animals

    Our Reduction Campaign

    At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...