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Mel624 (melanoma)

Description


Mel624 is a human malignant melanoma cell line. The cells are adherent and have an epithelial morphology. Mel624 cells will form tumors post-implantation into immunocompromised mice.

Usage Information:


Mel624 cells are suitable for in vitro and in vivo experimentation. Immunocompromised mice should be used for in life studies, and will form tumors following implantation of the cells.

The following chart provides from a study using Mel624 cells as a xenograft model.

Route of Implantation Mice Tumor/Metastases References
Subcutaneous NSG Subcutaneous tumor
Zheng et al. (2016) Oncotarget 7: 6448-6459
Tail vein NSG Nonspecific metastases
Zheng et al. (2016) Oncotarget 7: 6448-6459
Note: The above information is based on available data from the indicated references. It is not meant to be comprehensive and Imanis has not directly tested each condition.

Stable reporter cell lines:


Our Mel624 reporter cell lines can be tracked in vivo, making them great tools for studying the mechanisms of tumor growth and metastasis, as well as evaluating the effects of various drugs or therapies in animals. Our Mel624 cells are available with the human sodium iodide symporter (hNIS) alone or in combination with enhanced green fluorescent protein (eGFP) to facilitate multi-modality imaging.

In order to ensure high, constitutive expression of the reporter proteins, our cell lines are generated by lentivirus transduction. The lentivirus vectors used for these transductions are self-inactivating (SIN) vectors in which the viral enhancer and promoter has been deleted. This increases the biosafety of the lentiviruses by preventing mobilization of replication competent viruses (Miyoshi et al., J Virol. 1998).

What people say about Imanis

Our group has, and continues to, use NIS as a noninvasive reporter for cell transplantation studies in mice and in pigs. Imanis has provided expert technical and analytical support for this research, and has allowed us to publish our research in high impact journals, including Science Translational Medicine..

– Dr. Raymond Hickey, Mayo Clinic

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