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HT1080 (fibrosarcoma)

Description


HT1080 (ATCC® CCL-121™) is a human fibrosarcoma cell line initiated from a 35-year-old Caucasian male. The cells are adherent with an epithelial morphology. Following implantation into immunocompromised mice, the cells form primary tumors and distant metastases.

Usage Information:


HT1080 cells are suitable for in vitro and in vivo experimentation. Immunocompromised mice should be used for in life studies, and will form tumors and metastases following implantation of the cells.

The following chart provides some examples of HT1080 cells used as a xenograft model.

Route of Implantation Mice Tumor/Metastases References
Intramuscular Nude Primary tumor, lung metastases
Miwa et al. (2014) Oncotarget 5: 12849-12861.
Tail-vein Nude Lung metastases
Hanyu et al. (2009) Cancer Science 100: 2085-2092.
Fuse et al. (2007) J Biol Chem 282:8276-8283.
Subcutaneous Immunosupressed Subcutaneous tumor
Rasheed et al. (1974) Cancer 33:1027-1033.
Subcutaneous Nude Subcutaneous tumor
Shi et al. (2015) Int J Mol Med 35: 31-38.
Subcutaneous Nude Subcutaneous tumor, lung metastases, few liver and brain metastases
Praus et al. (1999) Gene Therapy 6: 227-236.
Mammary gland Nude Mammary tumor, lung metastases
Hanyu et al. (2009) Cancer Science 100: 2085-2092.
Note: The above information is based on available data from the indicated references. It is not meant to be comprehensive and Imanis has not directly tested each condition.

Stable reporter cell lines:


Our HT1080 reporter cell lines can be tracked in vivo, making them great tools for studying the mechanisms of tumor growth, as well as evaluating the effects of various drugs or therapies in animals. Our HT1080 cells are available with a variety of different reporters, including the human sodium iodide symporter (hNIS), firefly luciferase (Fluc), enhanced green fluorescent protein (eGFP), or near-infrared fluorescent protein (iRFP). Several dual reporter HT1080 cell lines are available to facilitate multi-modality imaging.

In order to ensure high, constitutive expression of the reporter proteins, our cell lines are generated by lentivirus transduction. The lentivirus vectors used for these transductions are self-inactivating (SIN) vectors in which the viral enhancer and promoter has been deleted. This increases the biosafety of the lentiviruses by preventing mobilization of replication competent viruses (Miyoshi et al., J Virol. 1998).

What people say about Imanis

Our group has, and continues to, use NIS as a noninvasive reporter for cell transplantation studies in mice and in pigs. Imanis has provided expert technical and analytical support for this research, and has allowed us to publish our research in high impact journals, including Science Translational Medicine..

– Dr. Raymond Hickey, Mayo Clinic

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