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A549 (lung adenocarcinoma)

Description


A549 (ATCC® CCL-185™) is a human lung adenocarcinoma cell line initiated through explant culture of lung carcinomatous tissue from a 58-year-old Caucasian male. The cells are adherent with an epithelial morphology. Following implantation into immunocompromised mice, the cells form primary tumors and pulmonary metastases.

Usage Information:


A549 cells are relatively large cells, with a doubling time of approximately 24 hours. They are suitable for in vitro and in vivo experimentation. Immunocompromised mice should be used for in life studies, and will form tumors and metastases following implantation of the cells.

The following chart provides some examples of A549 cells used as a xenograft model.

Route of Implantation Mice Tumor/Metastases References
Tail-vein Nude Lung metastases
Wang et al. (2014) Cancer Gene Therapy 21:150-157.
Jiang et al (2001) Oncogene 20:2254-2263.
Tail-vein SCID Lung metastases
Oleinik et al. (2014) J Biol Chem 289:26383-26394.
Jenkins et al. (2003) Clin & Exp Metastasis 20:733-744.
Intracardiac NOD/SCID Bone metastases
Hung et al. (2014) Laboratory Investigation 94: 371-381.
Subcutaneous Nude Subcutaneous tumor, few lung metastases
Jakubowka et al. (2013) ACTA Biochimica Polonica 60: 323-330.
Jiang et al (2001) Oncogene 20:2254-2263.
Intrathoracic Nude Lung metastases
Liu et al. (2012) J Thorac Dis 4:141-145
Note: The above information is based on available data from the indicated references. It is not meant to be comprehensive and Imanis has not directly tested each condition.

Stable reporter cell lines:


Our A549 reporter cell lines can be tracked in vivo, making them great tools for studying the mechanisms of tumor growth and metastasis, as well as evaluating the effects of various drugs or therapies in animals. Our A549 cells are available with a variety of different reporters, including the human sodium iodide symporter (hNIS), firefly luciferase (Fluc), enhanced green fluorescent protein (eGFP), or near-infrared fluorescent protein (iRFP). Several dual reporter A549 cell lines are available to facilitate multi-modality imaging.

In order to ensure high, constitutive expression of the reporter proteins, our cell lines are generated by lentivirus transduction. The lentivirus vectors used for these transductions are self-inactivating (SIN) vectors in which the viral enhancer and promoter has been deleted. This increases the biosafety of the lentiviruses by preventing mobilization of replication competent viruses (Miyoshi et al., J Virol. 1998).

What people say about Imanis

Our group has, and continues to, use NIS as a noninvasive reporter for cell transplantation studies in mice and in pigs. Imanis has provided expert technical and analytical support for this research, and has allowed us to publish our research in high impact journals, including Science Translational Medicine..

– Dr. Raymond Hickey, Mayo Clinic

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