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A375 (melanoma)

Description


A375 (ATCC® CCL-121™) is a human melanoma cell line initiated through explant culture of a solid tumor from a 54-year-old female. The cells are adherent with an epithelial morphology. The cells form tumors following implantation into immunocompromised mice.

Usage Information:


A375 cells are suitable for in vitro and in vivo experimentation. Immunocompromised mice should be used for in life studies, and will form tumors following implantation of the cells.

The following chart provides some examples of A375 cells used as a xenograft model.

Route of Implantation Mice Tumor/Metastases References
Intradermal NOD/SCID Dermal tumors, micrometastases in lung and brain
Rozenberg et al. (2010) Melanoma Res 20:361-371.
Subcutaneous Nude Subcutaneous tumor
White et al. (2009) Purinergic Signal 5: 327-333.
Tail vein NSG Lung metastases
Weber et al. (2016) Cancer Res 15: 3562-3571.
Intracardiac Nude Lung, liver, bone, and lymph node metastases
Huang et al. (2014) Endocrinology 155:3739-3749.
Note: The above information is based on available data from the indicated references. It is not meant to be comprehensive and Imanis has not directly tested each condition.

Stable reporter cell lines:


Our A375 reporter cell lines can be tracked in vivo, making them great tools for studying the mechanisms of tumor growth and metastasis, as well as evaluating the effects of various drugs or therapies in animals. Our A375 cells are available with a variety of different reporters, including the human sodium iodide symporter (hNIS), firefly luciferase (Fluc), enhanced green fluorescent protein (eGFP), or near-infrared fluorescent protein (iRFP). Several dual reporter A375 cell lines are available to facilitate multi-modality imaging.

In order to ensure high, constitutive expression of the reporter proteins, our cell lines are generated by lentivirus transduction. The lentivirus vectors used for these transductions are self-inactivating (SIN) vectors in which the viral enhancer and promoter has been deleted. This increases the biosafety of the lentiviruses by preventing mobilization of replication competent viruses (Miyoshi et al., J Virol. 1998).

What people say about Imanis

Our group has, and continues to, use NIS as a noninvasive reporter for cell transplantation studies in mice and in pigs. Imanis has provided expert technical and analytical support for this research, and has allowed us to publish our research in high impact journals, including Science Translational Medicine..

– Dr. Raymond Hickey, Mayo Clinic

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