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4T1 (mammary carcinoma)

Description


4T1 (ATCC® CRL-2539™) is a murine mammary carcinoma cell line from a BALB/cfC3H mouse. The cells are adherent and have a epithelial morphology. 4T1 cells will form tumors and spontaneous metastases post implantation into syngenic BALB/c mice or immunocompromised mice, which very closely mimic stage IV human breast cancer.

Usage Information:


4T1 cells are suitable for in vitro and in vivo experimentation. Both syngenic BALB/c mice and immunocompromised mice can be used for in life studies, and will form tumors and spontaneous metastases following implantation of the cells. Depending on the route of inoculation (see below), implanted 4T1 cells can metastasize to the lung, liver, lymph nodes, and brain, and micro-metastatic cells can be detected in many distant organs.

The following chart provides some examples of 4T1 cells used for tumor formation and studies.

Route of Implantation Mice Tumor/Metastases References
Subcutaneous BALB/c Subcutaneous tumor, lung metastases
Filatenkov et al. (2014) Radiation Res 182: 163-169.
Intracardiac BALB/c Bone metastases
Lee et al. (2014) Biochem Pharm 91: 51-60.
Zhang et al. (2012) Cancer Gen Therapy 19:630-636.
Orthotopic BALB/c Mammary tumor, Bone and lung metastases
Michigami et al. (2002) Breast Cancer Res Treat 75: 249-258.
Orthotopic Nude Mammary tumor, lung metastases
Wang et al. (2014) Biomaterials 35: 8374-8384.
Subcutaneous (mammary gland) BALB/c Mammary tumor, lung, liver, lymph node, blood, and brain metastases
Pulaski and Ostrand-Rosenberg (1998) Cancer Res 58:1486-1493.
Tail vein BALB/c Lung metastases
Thion et al. (2015) J National Cancer Institute 107
Jiang et al. (2014) Cancer Biol Ther 15: 789-796.
Note: The above information is based on available data from the indicated references. It is not meant to be comprehensive and Imanis has not directly tested each condition.

Stable reporter cell lines:


Our 4T1 reporter cell lines can be tracked in vivo, making them great tools for studying the mechanisms of tumor growth and metastasis, as well as evaluating the effects of various drugs or therapies in animals. Our 4T1 cells are available with a variety of different reporters, including the murine sodium iodide symporter (mNIS), firefly luciferase (Fluc), enhanced green fluorescent protein (eGFP), or near-infrared fluorescent protein (iRFP). Several dual reporter 4T1 cell lines are available to facilitate multi-modality imaging.

In order to ensure high, constitutive expression of the reporter proteins, our cell lines are generated by lentivirus transduction. The lentivirus vectors used for these transductions are self-inactivating (SIN) vectors in which the viral enhancer and promoter has been deleted. This increases the biosafety of the lentiviruses by preventing mobilization of replication competent viruses (Miyoshi et al., J Virol. 1998).

What people say about Imanis

Our group has, and continues to, use NIS as a noninvasive reporter for cell transplantation studies in mice and in pigs. Imanis has provided expert technical and analytical support for this research, and has allowed us to publish our research in high impact journals, including Science Translational Medicine..

– Dr. Raymond Hickey, Mayo Clinic

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