Reporter Gene Cell Lines
Imanis offers a wide-range of ready-to-use reporter cell lines for noninvasive imaging. Our expanding catalog includes multiple human and mouse cancer cell line models stably expressing a variety of reporter genes, giving you more options to best meet your research needs!
- A375 (melanoma)
- A549 (lung adenocarcinoma)
- HCT116 (colorectal carcinoma)
- HT1080 (fibrosarcoma)
- Mel624 (melanoma)
Better Reporter Gene Expression
Our stable reporter gene cell lines are generated by high-efficiency lentiviral vector transduction; the reporter gene is integrated in the target cell genome, facilitating stable reporter gene expression during continued passaging. Our polyclonal cell lines couple our reporter genes to an antibiotic selection gene (either puromycin or neomycin) to ensure that the population is comprised of high reporter gene-expressing cells. Our monoclonal cell lines undergo several rounds of selection to optimize high reporter gene expression. Reporter gene expression in each of our cell lines is driven by the spleen focus-forming virus (SFFV) promoter, which results in high constitutive expression in a wide range of mammalian cells and mouse models. Thus, our stable reporter gene cell lines are perfectly suited for in vivo imaging studies, where the high reporter gene expression facilitates detection of fewer implanted cells.
Imanis offers more options for in vivo imaging due to our unique selection of reporter genes. Our cancer cell models are available with one, or a combination, of our four main reporters: enhanced green fluorescent protein (eGFP), firefly luciferase (Fluc), near-infrared fluorescent protein (iRFP), or the sodium-iodide symporter (NIS). These reporter genes offer the flexibility to choose the imaging modality that will best meet your needs and equipment, whether it be intravital or conventional microscopy using eGFP, noninvasive in-life imaging using Fluc or iRFP, or noninvasive, high-resolution 3D PET/SPECT imaging using NIS.
Highest Quality Cells
Our cell lines undergo extensive quality control analyses so that you can choose Imanis with confidence. In addition to ensuring sterility, each cell line undergoes rigorous testing for reporter gene expression, so you get only the best reporter-expressing cell lines. All of our cell lines are certified mycoplasma free. Moreover, our parental cell lines are authenticated with short tandem repeat (STR) profiling, in accordance with recommendations by the NIH as well as several other funding organizations and major publishers*.