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A549 Cells

A549 Cells representative image
A549 Cells representative image

Description:

A549 (ATCC® CCL-185™) is a human lung adenocarcinoma cell line initiated through explant culture of lung carcinomatous tissue from a 58-year-old Caucasian male. The cells are adherent with an epithelial morphology. Following implantation into immunocompromised mice, the cells form primary tumors and pulmonary metastases.

Usage Information:

A549 cells are relatively large cells, with a doubling time of approximately 24 hours. They are suitable for in vitro and in vivo experimentation. Immunocompromised mice should be used for in life studies, and will form tumors and metastases following implantation of the cells.

The following chart provides some examples of A549 cells used as a xenograft model.

Route of Implantation

Mice

Tumor/Metastases

References

Tail-vein

Nude

Lung metastases

Wang et al. (2014) Cancer Gene Therapy 21:150-157.

Jiang et al (2001) Oncogene 20:2254-2263.

Tail-vein

SCID

Lung metastases

Oleinik et al. (2014) J Biol Chem 289:26383-26394.

Jenkins et al. (2003) Clin & Exp Metastasis 20:733-744.

Intracardiac

NOD/SCID

Bone metastases

Hung et al. (2014) Laboratory Investigation 94: 371-381.

Subcutaneous

Nude

Subcutaneous tumor, few lung metastases

Jakubowka et al. (2013) ACTA Biochimica Polonica 60: 323-330.

Jiang et al (2001) Oncogene 20:2254-2263.

Intrathoracic

Nude

Lung metastases

Liu et al. (2012) J Thorac Dis 4:141-145

Note: The above information is based on available data from the indicated references. It is not meant to be comprehensive and Imanis has not directly tested each condition.

Stable reporter cell lines: 

Our A549 reporter cell lines can be tracked in vivo, making them great tools for studying the mechanisms of tumor growth and metastasis, as well as evaluating the effects of various drugs or therapies in animals. Our A549 cells are available with a variety of different reporters, including the human sodium iodide symporter (hNIS), firefly luciferase (Fluc), enhanced green fluorescent protein (eGFP), or near-infrared fluorescent protein (iRFP). Several dual reporter A549 cell lines are available to facilitate multi-modality imaging.

In order to ensure high, constitutive expression of the reporter proteins, our cell lines are generated by lentivirus transduction. The lentivirus vectors used for these transductions are self-inactivating (SIN) vectors in which the viral enhancer and promoter has been deleted. This increases the biosafety of the lentiviruses by preventing mobilization of replication competent viruses (Miyoshi et al., J Virol. 1998). 

For more information about a specific reporter and its uses, click on the photos below.

Use Fewer Animals

Our Reduction Campaign

At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...

Use Fewer Animals

Our Reduction Campaign

At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...