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A375 Cells

A375 Cells representative image
A375 Cells representative image

Description:

A375 (ATCC® CCL-121™) is a human melanoma cell line initiated through explant culture of a solid tumor from a 54-year-old female. The cells are adherent with an epithelial morphology. The cells form tumors following implantation into immunocompromised mice.

Usage Information:

A375 cells are suitable for in vitro and in vivo experimentation. Immunocompromised mice should be used for in life studies, and will form tumors following implantation of the cells.

The following chart provides some examples of A375 cells used as a xenograft model.

Route of Implantation

Mice

Tumor/Metastases

References

Intradermal

NOD/SCID

Dermal tumors, micrometastases in lung and brain

Rozenberg et al. (2010) Melanoma Res 20:361-371.

Subcutaneous

Nude

Subcutaneous tumor

White et al. (2009) Purinergic Signal 5: 327-333.

Tail vein

NSG

Lung metastases

Weber et al. (2016) Cancer Res 15: 3562-3571.

Intracardiac

Nude

Lung, liver, bone, and lymph node metastases

Huang et al. (2014) Endocrinology 155:3739-3749.

Note: The above information is based on available data from the indicated references. It is not meant to be comprehensive and Imanis has not directly tested each condition.

Stable reporter cell lines: 

Our A375 reporter cell lines can be tracked in vivo, making them great tools for studying the mechanisms of tumor growth and metastasis, as well as evaluating the effects of various drugs or therapies in animals. Our A375 cells are available with a variety of different reporters, including the human sodium iodide symporter (hNIS), firefly luciferase (Fluc), enhanced green fluorescent protein (eGFP), or near-infrared fluorescent protein (iRFP). Several dual reporter A375 cell lines are available to facilitate multi-modality imaging.

In order to ensure high, constitutive expression of the reporter proteins, our cell lines are generated by lentivirus transduction. The lentivirus vectors used for these transductions are self-inactivating (SIN) vectors in which the viral enhancer and promoter has been deleted. This increases the biosafety of the lentiviruses by preventing mobilization of replication competent viruses (Miyoshi et al., J Virol. 1998). 

For more information about a specific reporter and its uses, click on the photos below.

Use Fewer Animals

Our Reduction Campaign

At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...

Use Fewer Animals

Our Reduction Campaign

At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...