What packaging system should I use with your plasmids to generate lentivirus?
Our lentiviruses are 2nd generation; our lentiviral transfer plasmids are meant to be used in a 3 plasmid 2nd generation packaging system:
- Lentiviral transfer plasmid – the lentiviral plasmid encoding your transgene (ie. pLV-SFFV-hNIS)
- Packaging plasmid - encoding Gag, Pol, Rev, and Tat. Tat is essential for 2nd generation packaging.
- Envelope plasmid – encoding the envelope protein (usually VSV-G)