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What generation are your lentiviruses? Why does generation matter?

Our lentiviruses are 2nd generation. Generation matters when considering which packaging plasmids to use to produce the lentivirus particles. Briefly, our lentiviral transfer plasmids are meant to be used in a 3 plasmid 2nd generation packaging system:

  1. Lentiviral transfer plasmid – the lentiviral plasmid encoding your transgene (ie. pLV-SFFV-hNIS)
  2. Packaging plasmid - encoding Gag, Pol, Rev, and Tat. Tat is essential for 2nd generation packaging.
  3. Envelope plasmid – encoding the envelope protein (usually VSV-G) 

3rd generation lentiviruses encode Gag/Pol and Rev on 2 separate plasmids and the transfer plasmids are Tat independent. Thus 2nd generation transfer plasmids (Imanis products) CANNOT be packaged by 3rd generation systems since 3rd generation systems do not include Tat expression.

Use Fewer Animals

Our Reduction Campaign

At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...

Use Fewer Animals

Our Reduction Campaign

At Imanis, we are committed to promoting the practice of the 3Rs in animal research. Learn how we are decreasing the use of animals and research as well as saving up to 15% on your orders. Continue reading...