What generation are your lentiviruses? Why does generation matter?
Our lentiviruses are 2nd generation. Generation matters when considering which packaging plasmids to use to produce the lentivirus particles. Briefly, our lentiviral transfer plasmids are meant to be used in a 3 plasmid 2nd generation packaging system:
- Lentiviral transfer plasmid – the lentiviral plasmid encoding your transgene (ie. pLV-SFFV-hNIS)
- Packaging plasmid - encoding Gag, Pol, Rev, and Tat. Tat is essential for 2nd generation packaging.
- Envelope plasmid – encoding the envelope protein (usually VSV-G)
3rd generation lentiviruses encode Gag/Pol and Rev on 2 separate plasmids and the transfer plasmids are Tat independent. Thus 2nd generation transfer plasmids (Imanis products) CANNOT be packaged by 3rd generation systems since 3rd generation systems do not include Tat expression.